Diazyme's N-Acetyl-β-D-glucosaminidase (NAG) Assay is ready-to-use for manual methods and adaptable for analyzers. The assay is specific for NAG and has excellent sensitivity with a linear range up to 200 IU/L² and excellent precision with a CV% of <5%.
Product | Catalog Number | Format | Method |
---|---|---|---|
Kit | DZ062A | R1/R2/R3 (Three Vial Liquid Stable) | Three Vial Liquid Stable, Colorimetric |
Calibrator | DZ062A-CAL | Cal: 1 Level (Lyophilized) | |
Control | DZ062A-CON | Con: 2 Level (Lyophilized) |
Product Features
Diazyme's N-Acetyl-β-D-glucosaminidase (NAG) Assay is ready-to-use for manual methods and adaptable for analyzers. The assay is specific for NAG and has excellent sensitivity with a linear range up to 200 IU/L² and excellent precision with a CV% of <5%. There is no significant interference from hemoglobin or albumin. Interference from bilirubin occurs only at levels higher than 5 mg/dL.
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Assay Principle
The reagents of the assay kit are in stable liquid formulation that allows ease of use coupled with enhanced performance characteristics. NAG hydrolyses 2-methoxy-4-(2'nitrovinyl)-phenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (MNP-GlcNAc) to 2-methoxy-4-(2'-nitrovinyl)-phenol product. The product formation is detected by development of color at 505nm upon addition of an alkaline buffer.
Intended use
The N-Acetyl-β-D-glucosaminidase (NAG) assay kit is for determination of NAG in urine samples. For Research Use Only in the USA.