Diazyme's enzymatic assay for glycated serum protein provides improved specificity and reliability compared to conventional NBT-based methods. The assay is highly precise with a total CV ≤ 1.3%. The assay offers extensive linearity up to 1354 μmol/L.
Product | Catalog Number | Format | Method |
---|---|---|---|
Kit | DZ112B | R1/R2 (Dual Vial Liquid Stable) | Dual Vial Liquid Stable, Enzymatic |
Calibrator | DZ112B-CAL | Cal: 2 Level (Lyophilized) | |
Control | DZ112B-CON | Con: 2 Level (Lyophilized) |
Product Features
Diazyme's enzymatic assay for glycated serum protein provides improved specificity and reliability compared to conventional NBT-based methods. The assay is highly precise with a total CV ≤ 1.3%. The assay offers extensive linearity up to 1354 μmol/L. The assay has no significant interference from: ascorbic acid (5 mg /dL); bilirubin (7.5 mg/dL); bilirubin conjugated (5 mg/dL); glucose (2400 mg/dL; hemoglobin (200 mg/dL); triglycerides (2000 mg/dL); and uric acid (35 mg/dL).
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Informational Articles
- An improved enzymatic assay for glycated serum protein
- A1C Combined With Glycated Albumin Improves Detection of Prediabetes in Africans: The Africans in America Study
- Analytical Evaluation of the Diazyme Glycated Serum Protein Assay on the Siemens ADVIA 1800: Comparison of Results Against HbA1c for Diagnosis and Management of Diabetes
Assay Principle
Diazyme's Glycated Serum Protein Assay uses proteinase K to digest GSP into low molecular weight glycated protein fragments (GPF), and uses Diazyme's specific fructosaminase™, a microorganism originated amadoriase to catalyze the oxidative degradation of Amadori product GPF to yield PF or amino acids, glucosone and H2O2. The H2O2 released is measured by a colorimetric Trinder end-point reaction. The absorbance at 546-600 nm is proportional to the concentration of glycated serum proteins.
![Glycated Serum Protein (Glycated Albumin) graph](../../../../content/images/products/DZ112B.gif)
Intended use
Diazyme Glycated Serum Protein Assay in conjunction with Diazyme Glycated Serum Protein single calibrator is intended for the quantitative determination of glycated serum proteins (GSP; glycated albumins; fructosamine) in serum. The measurement of glycated serum proteins is useful for monitoring diabetic patients. For in vitro diagnostic use only.
Regulatory Status
![FDA 510K Cleared](../../../../content/images/products/fda-510k-cleared.png)
![CE](../../../../content/images/products/ce-logo.png)